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Characterization of L-Asparaginase
Ahmed M. Abdel-Azeem
(Author)
·
Bassem A. Balbool
(Author)
·
Mohamed H. Moubasher
(Author)
·
LAP Lambert Academic Publishing
· Paperback
Characterization of L-Asparaginase - Balbool, Bassem A. ; Abdel-Azeem, Ahmed M. ; Moubasher, Mohamed H.
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Synopsis "Characterization of L-Asparaginase"
The presented study aims to the screening of the endophytic fungi associated with Saint Katherine plants to produce L-asparaginase enzyme. Twenty-five endophytic fungal species were isolated and identified from a twenty-three-plant species using surface sterilization techniques. Plant species were collected from nine wadis over Saint Katherine protectorate (SKP), during the period of 2015-2017 with a total count of 4466 CFU. Five species with nine morphotypes showed L-asparaginase activity ranging from 44.5 ( 1.66) to 152.58 ( 0.63) U/ml. Lasiodiplodia theobromae recoded the highest activity with 152.58 U/ml. L-asparaginase was induced with an activity of 315 U/ml. L¬-asparaginase was purified with a specific activity of 468.03 U/mg and a total activity of 84.4 U/ml. Purified L-asparaginase showed an approximate size of 70 kDa. Purified enzyme was characterized chemically. Purified L-asparaginase showed an IC50 of 35.2 0.7 U/ml with leukemic M-NFS-60 cell lines and CC50 of 79.4 1.9 U/ml with normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for L-asparaginase production.
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The book is written in English.
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