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portada Synthesis of tyrosinase by streptomyces SP.
Type
Physical Book
Language
Inglés
Pages
88
Format
Paperback
Dimensions
22.9 x 15.2 x 0.5 cm
Weight
0.14 kg.
ISBN13
9786207473359

Synthesis of tyrosinase by streptomyces SP.

Raghavendra H (Author) · LAP Lambert Academic Publishing · Paperback

Synthesis of tyrosinase by streptomyces SP. - H, Raghavendra

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Synopsis "Synthesis of tyrosinase by streptomyces SP."

- In all 15 isolates of Streptomyces were screened for the synthesis of tyrosinase by both qualitative and quantitative methods in synthetic medium. - Activity of tyrosinase, specific activity of tyrosinase, formation of melanin and protein content of tyrosinase were assessed to determine the catalytic power of tyrosinase. - In a batch wise bioprocess, an isolate of Streptomyces DLS-8 has been found to be potential for the production of tyrosinase. - Effect of pH and temperature on the production of tyrosinase were also examined. 300C temperature and pH 8.5 were proved to be optimum for the maximum production of tyrosinase. - Results of the present study revealed that starch and citrulline were the important carbon and nitrogen sources respectively for the maximum production of tyrosinase. - The partial isolation and purification of the tyrosinase was under taken by SDS-PAGE. The tyrosinase was determined to be about 45 KDa, comparing to BSA as standard marker.- Liquid chromatogram mass spectrum reveals the presence of tyrosine, L-Dopa and melanin based on their base peaks for their respective molecular weights indicating significantly the catalytic power of tyrosinase.

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